Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00157
OS Name
Optogenetic LaM4 (OptoLaM4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein A anti-mCherry nanobody
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Nanobody binds to respective binder
Effect Activation
Application Optogentic control protein binding using light switchable nanobodies
Note Open the door to developing light-switchable binders against a broad range of protein targets and may thus represent a first step toward an important class of photoswitchable biologics
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 2 min
Deactivation Time Minutes
Light Intensity 1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines NIH 3T3 cells
References
1 Optogenetic control of protein binding using light-switchable nanobodies