Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00056
|
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| OS Name |
Exosomes for protein loading via optically reversible proteinCprotein interactions system
(EXPLORs)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Functional protein | Cryptochrome-2 | ||||
| Recruited protein | Exosome-associated tetraspanin protein CD9 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Exocytosis and endocytosis | |||||
| Effect | Activation | |||||
| Application | Deliver intracellular protein via a reversible protein-protein interactions | |||||
| Note | mCherry as cargo protein and can be changed or taged to other protein for application | |||||
| Light Information | Activation Wavelength | 488 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 15 s | |||||
| Light Intensity | 20C50 microwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells and Hela cells | |||||