Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00049
OS Name
Optogenetic regulate iTrkA activity by CRY2 system (Opto-iTrkA-CRY2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Tropomyosin receptor kinases A
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway TrkA signal activation
Effect Activation
Application Light-dependent protein translocation to activate iTrkA by CRY2
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 9 watts per square centimeter; 400 microwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC12 cells
References
1 Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID)