Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00038
OS Name
Optogentic regulate stromal interaction molecule 1 system 4 (OptoSTIM1-4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Stromal interaction molecule 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway STIM1-gated Ca2+ influxion
Effect Activation
Application CRY2PHR oligomerization to disrupt CC1-SOAR interaction and subsequently trigger Ca2+ influx
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 4.0 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling