Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00036
OS Name
Optogentic regulate stromal interaction molecule 1 system 1 (OptoSTIM1-1)
 [1], [2], [3]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Stromal interaction molecule 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homo-oligomerization [ 1]
Controlled Signal Pathway Protein cluster and CRAC channel open
Effect Activation
Application Activation of Ca2+ selective channel to manipulate intracellular Ca2+ levels
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 148 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines Zebrafish embryos or Human embryonic stem cells
OS Application Information (2)
Mechanism of Action CRY2 homo-oligomerization [ 3]
Controlled Signal Pathway Ca2+ release
Effect Activation
Application PGE2 discharge and increase cytoplasmic Ca2+ concentrations
Light Information Activation Wavelength 475 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 200 ms
Deactivation Time 30 min
Light Intensity 0.8 to 67 microwatts per square milimeter
Expression Information Expression Method Transfection
Cell Lines MDCK cells
References
1 Optogenetic control of endogenous Ca(2+) channels in vivo
2 Visual quantification of prostaglandin E(2) discharge from a single cell
3 Calcium transients trigger switch-like discharge of prostaglandin E2in an extracellular signal-regulated kinase-dependent manner