Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00033
OS Name
Optogenetic regulated 5-photolysase system (Opto-5-ptase)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Inositol-polyphosphate-5-phosphatase OCRL
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIB1 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Phosphoinositide metabolism to control cellular function
Note Can be modified by targeting different phosphoinositides metabolizing enzymes to various cellular membranes
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 50 milliwatts
Expression Information Expression Method Transfection
Cell Lines COS-7 cells
OS Application Information (2)
Mechanism of Action CRY2PHRCCIBN heterodimerization [ 2]
Controlled Signal Pathway Regulate apical constriction during tissue morphgenesis
Effect Inhibition
Application Optogenetic inhibition of constriction during drosophila embryogenesis
Note Actin-dependent morphogenetic processes
Light Information Activation Wavelength 488 nm and 950 nm for two proton stimulation
Activation Time Seconds (one proton) or Subminutes (two proton)
Light Intensity 6.9 W(one proton) or 3 mW(two proton)
Expression Information Expression Method Transgene
Cell Lines Drosophila embryos
References
1 Optogenetic control of phosphoinositide metabolism
2 Optogenetic inhibition of apical constriction during Drosophila embryonic development