Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00027
OS Name
Optogenetic controlled GEF signaling to regulate Ccd42 system (OptoGEF-Cdc42)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein Intersectin (ITSN) guanine exchange factor (GEF) catalytic domain
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Cdc42 activation
Effect Activation
Application Optogenetic control Cdc42 Rho GTPase activity
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 4C20 microwatts
Expression Information Expression Method Transfection
Cell Lines NIH 3T3 cells
References
1 Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics