Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00728
OS Name
Optogenetics system activates by BcWCL1PAS 1 (Opto-BcWCL1PAS 1)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
BcWCL1PAS (BcWCL1PAS)
Source of Species
Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea)
Class
LOV domain
UniProt ID
A0A384JAY1
3D Structure
Components
Photoreceptor: BcWCL1PAS (BcWCL1PAS)
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein DNA Binding Domain
Detail Info
OS Application Information (1)
Mechanism of Action BcWCL1PAS self-dimerization [ 1] , [ 2]
Controlled Signal Pathway Transcription
Effect Activation
Application Transcriptional activation in yeast
Note BcWCL1PAS AD can reach higher levels of transcriptional activation than those obtained with commonly used ADs
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time 2 h
Expression Information Expression Method Transformation
Cell Lines Yeast
References
1 The N-Terminal Region of the BcWCL1 Photoreceptor Is Necessary for Self-Dimerization and Transcriptional Activation upon Light Stimulation in Yeast
2 Interactions between Core Elements of theBotrytis cinereaCircadian Clock Are Modulated by Light and Different Protein Domains