Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00724
OS Name
Bue light-inducible nuclear export system - s 4 (iLEXYs 4)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
V416L
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (V416L) (AsLOV2 (V416L))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway DNA
Effect Sensor
Application Site-specific DNA recombinase
Note The mutations slow down the dark reversion rate
Light Information Activation Wavelength Blue
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 min
Deactivation Time t1/2 = 636.6 s
Expression Information Expression Method Transfection
Cell Lines HEK 293E cells
References
1 Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear Protein Export System LEXY in Mammalian Cells