Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00707
OS Name
Optogenetic system of peripheral somatosensory neurons (Opto-PSN)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Channelrhodopsin 2 (ChR2)
Source of Species
Chlamydomonas reinhardtii
Class
Opsin
UniProt ID
Q8RUT8
GenBank
1304111922
PDB ID
6EID
3D Structure
Components
Photoreceptor: Channelrhodopsin 2 (ChR2)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Channelrhodopsin 2
Detail Info
OS Application Information (1)
Controlled Signal Pathway Primary somatosensory neurons
Effect Activation
Application Mice nocifensive paw withdrawal behavior
Note NSAIDs have no effect on optogenetic-evoked paw withdrawal response; local anesthetic, but Not morphine, attenuates the light-evoked paw withdrawal response
Light Information Activation Wavelength 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 5 ms
Light Intensity 60 microwatts per square milimeter
Expression Information Expression Method Transgene
Cell Lines Mice primary somatosensory neurons
References
1 Optogenetic Activation of Peripheral Somatosensory Neurons in Transgenic Mice as a Neuropathic Pain Model for Assessing the Therapeutic Efficacy of Analgesics