Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00653
OS Name
Light-inducible cell reprogramming system (LIRE)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein LexA activation domain
Detail Info
Recruited protein Tetracycline repressor protein class D
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR-CIBN heterodimerization [ 1]
Controlled Signal Pathway Sox2 and Oct4 signaling
Effect Activation
Application Cell reprogramming and generation of induced pluripotent stem cells (iPSCs)
Note Enable non-invasive control of user-defined endogenous genes in vivo
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Expression Information Expression Method Transduction
Cell Lines Mouse embryonic fibroblasts (MEFs)
References
1 An Optogenetic-Controlled Cell Reprogramming System for Driving Cell Fate and Light-Responsive Chimeric Mice