Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00634
OS Name
Optogenetic bispecific T-cell engagers (OptoBiTEs)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Source of Species
Deinococcus radiodurans
Class
Phytochromes
UniProt ID
Q9RZA4
GenBank
134105080
PDB ID
6FHT
3D Structure
Components
Photoreceptor: Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Cofactor: Biliverdin (BV)
Cofactor Biliverdin (BV)
Controlled Protein Fusion protein Putative DNA binding protein
Detail Info
The bacterial c-di-GMP phosphodiesterase
Detail Info
OS Application Information (1)
Mechanism of Action c-di-GMP synthesis [ 1]
Controlled Signal Pathway Gene expression
Effect Activation
Application Optogenetics can regulate the production of BiTEs from bioengineered cells for the treatment of cancer
Note This method could be potentially applied to other formats of bispecific antibodies and facilitate their clinical applications
Light Information Activation Wavelength Far-red Light
Process Reversible
Deactivation Wavelength Dark
Activation Time 24 h
Light Intensity 1 miliwatt per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK293FT cells
References
1 Far-Red Light Triggered Production of Bispecific T Cell Engagers (BiTEs) from Engineered Cells for Antitumor Application