Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00629
|
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| OS Name |
Optogenetic mitochondira deformation system 1
(OptoMD 1)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Kinesin heavy chain isoform 5A | ||||
| Fusion protein | Transmembrane domain of Miro1 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Mitochondria deformation | |||||
| Effect | Activation | |||||
| Application | Light inducible deformation of mitochondria by recruiting molecular motors to the outer mitochrondrial membrane | |||||
| Note | Both the outer and inner membranes of mitochondria can be stretched and deformed using this optogenetic system | |||||
| Light Information | Activation Wavelength | Blue | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 20 s | |||||
| Deactivation Time | 70 s | |||||
| Light Intensity | 9.7 watts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | COS cells; HeLa cells; U2OS cells; 3T3 cells | |||||