Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00626
OS Name
Optogenetic multiple sclerosis mechanism researching system 3 (OptoMSMR 3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Heterogeneous ribonucleoprotein A1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 cluster [ 1]
Controlled Signal Pathway hnRNPA1 self-association
Effect Sensor
Application Mechanism analysis of A1 self-association dynamics and protein mislocalization in Multiple sclerosis
Note Mutations within the C-terminal prion-like domain of RNA binding proteins alter protein-protein interactions will lead to mislocalization and aggregate formation
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 120 min
Deactivation Time 60 min
Light Intensity 10000 lux
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Multiple Sclerosis-Associated hnRNPA1 Mutations Alter hnRNPA1 Dynamics and Influence Stress Granule Formation