Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00605
OS Name	Optogenetic control gene expression by green light (OptoGE-GL)		[1]
Photoreceptor (PR) PR Info	PR Name Cobalamin-binding domain from Thermus thermophilus (TtCBD) Source of Species Thermus thermophilus Class Cobalamin binding domain 3D Structure Components Photoreceptor: Cobalamin-binding domain from Thermus thermophilus (TtCBD) Cofactor: 5-deoxyadenosylcobalamin (AdoCbl)
Cofactor	5-deoxyadenosylcobalamin (AdoCbl)
Controlled Protein	Fusion protein	Tetracycline repressor protein class D	Detail Info
Controlled Protein	Recruited protein	Tetracycline repressor protein class D	Detail Info

OS Application Information (1)
Mechanism of Action	Dissociation		[ 1]
Controlled Signal Pathway	Gene expression
Effect	Activation
Application	Cure type-2 diebetes by producing and releasing human glucagon-like peptide-1 on demand
Note	Use smart-watch to emit green light
Light Information	Activation Wavelength	545 nm
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	15 s
	Light Intensity	88 microwatts per square centimeter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293T

References

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes