Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00601
OS Name
Optogenetic functional ultrasound imaging system (Opto-FUS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
ChrimsonR (ChrimsonR)
Source of Species
Synthetic construct
Class
Opsin
GenBank
AHH02126.1
Mutation Site
K176R
3D Structure
Components
Photoreceptor: ChrimsonR (ChrimsonR)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Chrimson
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Visual formation
Effect Sensor
Application Ultrafast functional ultrasound imaging (FUS) in deep tissue
Note ChrimsonR is K176R mutation from Chrimson and the firing rates induced by optogenetic stimulation were similar to those induced by visual stimulation
Channel Type Cation channel
Light Information Activation Wavelength 595 nm
Activation Time 2 s
Light Intensity 6 milliwatts per square miliimeter
Expression Information Expression Method Transfection
Cell Lines Rat cortex
References
1 Functional ultrasound imaging of the spreading activity following optogenetic stimulation of the rat visual cortex