Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00550
OS Name
Far-red light-induced split Cre-LoxP system (FISC)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Engineered high-potency photoactivated DGC (BphS)
Source of Species
Rhodobacter sphaeroides
Class
Phytochromes
3D Structure
Components
Photoreceptor: Engineered high-potency photoactivated DGC (BphS)
Cofactor: Biliverdin (BV)
Cofactor Biliverdin (BV)
Controlled Protein Fusion protein The bacterial c-di-GMP phosphodiesterase
Detail Info
OS Application Information (1)
Mechanism of Action c-di-GMP hydrolysis [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Far red light induced split-Cre recombinase system for controllable genome engineering
Note Low background and no detectable photocytotoxity and the controlld protein (Cre) express in the downstream
Light Information Activation Wavelength 730 nm
Activation Time Hours
Light Intensity 1.5 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK239 cells
References
1 A non-invasive far-red light-induced split-Cre recombinase system for controllable genome engineering in mice