Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00549
OS Name
Nanobody-based red light-induced dimerization 3 (NanoReD 3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Source of Species
Deinococcus radiodurans
Class
Phytochromes
UniProt ID
Q9RZA4
GenBank
134105080
PDB ID
6FHT
3D Structure
Components
Photoreceptor: Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Cofactor: Biliverdin (BV)
Cofactor Biliverdin (BV)
Controlled Protein Functional protein Cryptochrome-2
Detail Info
Recruited protein Light-induced dimerization binder-3
Detail Info
OS Application Information (1)
Mechanism of Action Comformational change to bind a dimerization binder [ 1]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Red light switchable photoreceptor and nanobody to control protein location
Note Nanobody as binder to bind to respective tag toactivate transcription
Light Information Activation Wavelength 654 nm
Process Reversible
Deactivation Wavelength 775 nm
Activation Time 2 min
Deactivation Time 10 min
Light Intensity 0.2 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity