Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00546
OS Name
Light-activated phosphodiesterases (LAPD)
 [1], [2], [3]
Photoreceptor (PR)
PR Info
PR Name
Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Source of Species
Deinococcus radiodurans
Class
Phytochromes
UniProt ID
Q9RZA4
GenBank
134105080
PDB ID
6FHT
3D Structure
Components
Photoreceptor: Bacteriophytochrome from Deinococcus radiodurans (DrBphP (1-506))
Cofactor: Biliverdin (BV)
Cofactor Biliverdin (BV)
Controlled Protein Fusion protein cGMP-dependent 3',5'-cyclic phosphodiesterase
Detail Info
OS Application Information (1)
Mechanism of Action Comformational change [ 1] , [ 2] , [ 3]
Controlled Signal Pathway Hydrolysis cAMP/cGMP to interfere Ca2+ influx
Effect Inhibition
Application Optogenetic control cyclic sdenosine monophosphate (cAMP) in mammalian cells
Note No exogenous biliverdin was needed and LAPD could not be fully switched off by far-red light
Light Information Activation Wavelength 670 nm
Process Reversible
Deactivation Wavelength 780 nm
Activation Time 10 s
Deactivation Time 10 s
Light Intensity 500 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK-TM cells
References
1 Revisiting and Redesigning Light-Activated Cyclic-Mononucleotide Phosphodiesterases
2 Structure-guided design and functional characterization of an artificial red light-regulated guanylate/adenylate cyclase for optogenetic applications
3 Engineering of a red-light-activated human cAMP/cGMP-specific phosphodiesterase