Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00544
OS Name
Optogenetic TrkA regulate system (Opto-TrkA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Phytochrome B tetramer (PhyBt)
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Phytochrome B tetramer (PhyBt)
Complementary Protein: Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor Phycocyanobilin (PCB)
Controlled Protein Fusion protein Tropomyosin receptor kinases A
Detail Info
OS Application Information (1)
Mechanism of Action Comformational change [ 1]
Controlled Signal Pathway Elk-1; CREB; and c-Jun signaling activation
Effect Activation
Application Reversibily regulate the PI3K pathway; calcium signaling; induce neurite outgrowth and activate MAPK/ERK signaling and other cell signal regulated by TrK
Note The activation time and deactivation time varied between cell signal modulated
Light Information Activation Wavelength 780 nm
Process Reversible
Deactivation Wavelength 660 nm
Activation Time Seconds to Minutes
Deactivation Time Seconds to Minutes
Light Intensity 0.5 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines PC6-3 cells; NIH 3T3 cells; Hela cells and SH-SY5Y cells
References
1 Neurotrophin receptor tyrosine kinases regulated with near-infrared light