Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00536
OS Name
Optogenetic modify subcellular protein localization system (Opto-MSPL)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Residue1-621 of Phytochrome B (PhyB (1-621))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621))
Complementary Protein: Phytochrome interacting factor 6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Recruited protein Partitioning defective 3
Detail Info
Functional protein Phytochrome B
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Optical control subcellular protein location and signal
Light Information Activation Wavelength 633 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 6.5 s
Deactivation Time 46.9 s
Expression Information Expression Method Transfection
Cell Lines Zebrafish embryos
References
1 Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos
2 Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo