Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00526
OS Name	Optogenetic control transcription activation system 1 (Opto-CTA 1)		[1]
Photoreceptor (PR) PR Info	PR Name Phytochrome B (PhyB) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR Components Photoreceptor: Phytochrome B (PhyB) Complementary Protein: Phytochrome interacting factor 6 (PIF6) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 6 (PIF6)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Recruited protein	Phytochrome interacting factor 6	Detail Info
Controlled Protein	Fusion protein	Virus protein 16	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF6 heterodimerization		[ 1]
Controlled Signal Pathway	Gene expression activation
Effect	Activation
Application	Red-far red light to control gene expression in plant cells
Note	Green light illumination won't activate PhyB; Promoter: pA: polyadenylation signal; PCaMV35S: cauliflower mosaic virus 35S promoter; E: macrolide repressor DNA-binding protein
Light Information	Activation Wavelength	660 nm
	Process	Reversible
	Deactivation Wavelength	760 nm
	Activation Time	6 h
	Light Intensity	0.5-16 micromoles per square meter(660nm) 17 micromoles per square meter (760nm)
Expression Information	Expression Method	Transformation
Expression Information	Cell Lines	Leaf protoplasts

References

Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression