Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00523
|
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| OS Name |
Optogenetically regulate membrane receptor-ligand pair system
(Opto-MCL)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIF6(C9S/C10S/S37A)) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Functional protein | Phytochrome B | ||||
| Recruited protein | Phytochrome interacting factor 6 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | PhyB-PIFs heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Activation | |||||
| Application | Optically control the integrin cell surface receptors with the extracellular matrix | |||||
| Note | Potential to provide enprecendented insight into the dynamics and signalling sequelae of receptor activation | |||||
| Light Information | Activation Wavelength | 660 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 740 nm | |||||
| Activation Time | Minutes | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 300 micromoles per square meter per second (660nm); 2000 micromoles per square meter per second (740nm) | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells; Hela cells and MCF7 cells | |||||