Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00519
OS Name
NIR and blue light sensing system for control protein localization (IRIS)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
Source of Species
Rhodopseudomonas palustris
Class
Phytochromes
UniProt ID
A0A161I5N6
GenBank
1569047741
PDB ID
5OY5
3D Structure
Components
Photoreceptor: Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein: Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor: Biliverdin (BV)
Complementary Protein Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor Biliverdin (BV)
Controlled Protein Functional protein Bacterial phytochrome photoreceptors 1
Detail Info
Recruited protein LOV2 domain of Avena sativa Phototropin 1
Detail Info
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass
Detail Info
OS Application Information (1)
Mechanism of Action BphP1-Q-PAS1 heterodimerization and AsLOV2 Comformational change [ 1] , [ 2]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Light controlled protein location in mammalian cells
Note Allows tridirectional protein translocation among the cytoplasm, nucleus and plasma membrane.
Light Information Activation Wavelength 740 nm
Process Reversible
Deactivation Wavelength 460 nm
Activation Time 5-10 min
Deactivation Time Minutes
Light Intensity 1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells; U-2 OS cells; HEK293 cells; cos-7 cells;SH-SY5Y cells; Neuro-2a cells; NIH 3T3 cells
References
1 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
2 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells