Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00312
|
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| OS Name |
Luminopsin 3 induced pluripotent stem cell-derived neural progenitor cells system
(LMO3-iPS-NPC)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Cofactor | All-trans-Retinal (Trans-Retinal) | |||||
| Controlled Protein | Functional protein | Volvox channelrhodopsin-1 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Ion channel open | [ 1] | ||||
| Controlled Signal Pathway | Neuron activation | |||||
| Effect | Activation | |||||
| Application | Neural network reconnection for repairing damaged brain | |||||
| Note | Lumiopsin 3 (LMO3) is fusion of sbGLuc-VChR1-EYFP and the system can be activated by either photostimulation by light or by the luciferase substrate coelenterazine (CTZ) | |||||
| Current Intensity | 60 mV | |||||
| Channel Type | Cation channel | |||||
| Light Information | Activation Wavelength | 473 nm or CTZ | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 12 s | |||||
| Deactivation Time | 48 s | |||||
| Light Intensity | 33.6 milliwatts | |||||
| Expression Information | Expression Method | Transduction | ||||
| Cell Lines | Mouse iPS-NPCs | |||||