Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00311
OS Name
Optogenetic activate neuron by LMO 3 system (Opto-AN-LMO3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Volvox channelrhodopsin 1 (VChR1)
Source of Species
volvox
Class
Opsin
UniProt ID
B4Y103
GenBank
167650744
3D Structure
Components
Photoreceptor: Volvox channelrhodopsin 1 (VChR1)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Volvox channelrhodopsin-1
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Acivate neuron cell by applying a substrate Gluc coelenterazine (CTZ)
Note LMO3 is chimera of sbGLuc-VChR1 and sbGluc is M43L/M110L double mutant of Gluc
Current Intensity 993 ± 185 pA
Channel Type Cation channel
Light Information Activation Wavelength 488 nm or CTZ
Light Intensity 10 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines Rat substantia nigra neurons
References
1 Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation