Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00308
OS Name
Optogenetic fast neural control system (OptoFNC)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Volvox channelrhodopsin 1 (VChR1)
Source of Species
volvox
Class
Opsin
UniProt ID
B4Y103
GenBank
167650744
3D Structure
Components
Photoreceptor: Volvox channelrhodopsin 1 (VChR1)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Volvox channelrhodopsin-1
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Red shift optogenetic excitation can work as a tool for fast neural control
Note Evoked photocurrents similar to those of ChR1 from Chlamydomonas
Current Intensity 208.8 ± 22.3 pA
Channel Type Cation channel
Light Information Activation Wavelength 531 nm or 589 nm
Process Reversible
Activation Time 2.8 ± 0.3 ms
Deactivation Time 133.4 ± 11.7 ms
Light Intensity 15 milliwatts per square miliimeter(531nm);13.8 milliwatts per square miliimeter(589nm)
Expression Information Expression Method Transfection
Cell Lines Xenopus oocytes and HEK293 cells
OS Application Information (2)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Red shift optogenetic excitation can work as a tool for fast neural control
Note Evoked photocurrents similar to those of ChR1 from Chlamydomonas
Current Intensity 177.6 ± 24.7 pA
Channel Type Cation channel
Light Information Activation Wavelength 531 nm or 589 nm
Process Reversible
Activation Time 8 ± 0.7 ms
Deactivation Time 135.7 ± 9.8 ms
Light Intensity 15 milliwatts per square miliimeter(531nm);13.8 milliwatts per square miliimeter(589nm)
Expression Information Expression Method Transfection
Cell Lines Xenopus oocytes and HEK293 cells
References
1 Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri