Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00307
OS Name
Enhenced iteration of the excitory liminopsin LMO3 (ELMO3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Volvox channelrhodopsin 1 (VChR1)
Source of Species
volvox
Class
Opsin
UniProt ID
B4Y103
GenBank
167650744
3D Structure
Components
Photoreceptor: Volvox channelrhodopsin 1 (VChR1)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Volvox channelrhodopsin-1
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Wihisker related movement regulation
Effect Activation
Application Improved expression of luminopsins for more efficeient optical control neuronal activity
Note SS (secretion signal) is necessary for membrane targeting
Channel Type Cation channel
Light Information Activation Wavelength 460-500 nm or CTZ luminescence
Activation Time 45min for CTZ
Light Intensity 300 microwatts per square millimeter
Expression Information Expression Method Transfection
Cell Lines Male mouse barrel cortex
References
1 Improved trafficking and expression of luminopsins for more efficient optical and pharmacological control of neuronal activity