Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00297
OS Name
MCh tag Channelrhodopsin2 for neuron signal regulation system 2 (MCh-ChR2-NSR 2)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Channelrhodopsin 2 (ChR2)
Source of Species
Chlamydomonas reinhardtii
Class
Opsin
UniProt ID
Q8RUT8
GenBank
1304111922
PDB ID
6EID
3D Structure
Components
Photoreceptor: Channelrhodopsin 2 (ChR2)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Channelrhodopsin 2
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 2]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Optical control rodent motor cortex neurons
Current Intensity 120 mV
Channel Type Cation channel
Light Information Activation Wavelength 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 10 ms
Deactivation Time 250-300 ms
Light Intensity 10 milliwatts per square miliimeter
Expression Information Expression Method Transduction
Cell Lines Mouse layer 5 motor cortex
OS Application Information (2)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Optical deconstruction of parkinsonian neural circuitry
Note Cause Parkinson's disease
Current Intensity 0.01 mV
Channel Type Cation channel
Light Information Activation Wavelength 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 20 milliwatts
Expression Information Expression Method Transduction
Cell Lines Rat excitatory glutamatergic neurons in STN
References
1 Optical deconstruction of parkinsonian neural circuitry
2 An optical neural interface: in vivo control of rodent motor cortex with integrated fiberoptic and optogenetic technology