Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00295
OS Name
Optogenetic regulation VTA dlutamate neuron system (Opto-VTA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Channelrhodopsin 2 (ChR2)
Source of Species
Chlamydomonas reinhardtii
Class
Opsin
UniProt ID
Q8RUT8
GenBank
1304111922
PDB ID
6EID
3D Structure
Components
Photoreceptor: Channelrhodopsin 2 (ChR2)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Channelrhodopsin 2
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Neuron activation
Effect Activation
Application Study the VTA glutamate neurons modulate the firing rate of pyramidal neurons in the hippocampus of anesthetized mice
Channel Type Cation channel
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 10 ms
Deactivation Time Seconds
Light Intensity 21.8 milliwatts
Expression Information Expression Method Transfection
Cell Lines C57BL/6 mouse VTA glutamate neurons
References
1 Combined In Vivo Anatomical and Functional Tracing of Ventral Tegmental Area Glutamate Terminals in the Hippocampus