Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00294
OS Name
Optogenetic activation brown adipose tissue for sympathetic neuromodulation 1 (Opto-BAT-SN1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Channelrhodopsin 2 (ChR2)
Source of Species
Chlamydomonas reinhardtii
Class
Opsin
UniProt ID
Q8RUT8
GenBank
1304111922
PDB ID
6EID
3D Structure
Components
Photoreceptor: Channelrhodopsin 2 (ChR2)
Cofactor: All-trans-Retinal (Trans-Retinal)
Cofactor All-trans-Retinal (Trans-Retinal)
Controlled Protein Functional protein Channelrhodopsin 2
Detail Info
OS Application Information (1)
Mechanism of Action Ion channel open [ 1]
Controlled Signal Pathway Elicit BAT and core thermogenesis
Effect Activation
Application Elicit BAT and core thermogenesis for treatment of type 2 diabetes and obesity
Note BAT temperature decrease while the core temperature continued to rise when the lights off
Channel Type Cation channel
Light Information Activation Wavelength 455 nm
Activation Time 30 min
Light Intensity 10 milliwatts per square miliimeter
Expression Information Expression Method Transfection
Cell Lines Mouse sympathetic nerves
References
1 Optogenetic-induced sympathetic neuromodulation of brown adipose tissue thermogenesis