Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00256
OS Name
Blue liight-responsive optogenetic OFF switch (Blue-OFF)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein KRAB domain of a transrepressor domain
Detail Info
OS Application Information (1)
Mechanism of Action EL222 homodimerization and DNA binding [ 1] , [ 2]
Controlled Signal Pathway Translation inhibition
Effect Inhibition
Application Optogenetic system to down-regulate protein levels by inhibit transcription
Note Dual-control protein levels with B-LID
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time 11 s
Light Intensity 20 micromoles per square meter per second
Expression Information Expression Method Transfection
Cell Lines HEK-293T; HeLa cells; COS-7 cells and CHO-K1 cells and NIH 3T3 cells
References
1 Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells
2 Silencing of RNA polymerases II and III-dependent transcription by the KRAB protein domain of KOX1, a Krppel-type zinc finger factor