Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00255
OS Name
Zebrafish-optimized optogenetic gene expression system (TAEL)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein An optimized Gal4-activator
Detail Info
OS Application Information (1)
Mechanism of Action EL222 homodimerization and DNA binding [ 1] , [ 2]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application A zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control
Note Minimal toxicity while exhibiting a large range of induction; fine spatial precision and rapid kinetics
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 1.6 milliwatts per square centimeter
Expression Information Expression Method Transgene
Cell Lines Adult Danio rerio Zebrafish cells
References
1 TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control
2 Optimized Gal4 genetics for permanent gene expression mapping in zebrafish