Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00252
OS Name
Optogenetic CRISPR interference system (Opto-CRISPRi)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein EL222 operator
Detail Info
OS Application Information (1)
Mechanism of Action EL222 homodimerization and DNA binding [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Enhance chemical synthesis with broad utilities
Note Signal receiver plasmid for EGFP expression contains the EL222 binding site (GGTAGCCTTTAGTCCATG)
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 50 micromoles per square meter per second
Expression Information Expression Method Transformation
Cell Lines E. coli MG1655(DE3)
References
1 Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production