Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00251
OS Name
Blue light repressible promoter (PBLrep-v1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein RNA polymerase
Detail Info
OS Application Information (1)
Mechanism of Action EL222 bind to EL222 binding region and hindrance RNAP binding [ 1]
Controlled Signal Pathway Represion gene expression
Effect Inhibition
Application Blue light mediated repression of gene expression in bacteria
Note Express EL222 binding domain at consensus -35 and -10 regions to hindrance RNA polymerase bind
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 12 watts per square meter
Expression Information Expression Method Transformation
Cell Lines E. coli
References
1 Blue light-mediated transcriptional activation and repression of gene expression in bacteria