Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00249
OS Name
Optogenetic gene expression system (Opto-GE)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action EL222 homodimerization and DNA binding [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Optogenetic gene expression system with rapid kinetics
Note EL222=LOV-HTH (helix-turn-helix DNA-binding domain)
Light Information Activation Wavelength 465 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time <10 s
Deactivation Time <50 s
Light Intensity 8 watts per square meter
Expression Information Expression Method Transfection
Cell Lines Jurkat splicing line 1 cell lines
References
1 An optogenetic gene expression system with rapid activation and deactivation kinetics