Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00248
OS Name
Optogenetic control Rac1 system (Opto-Rac1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV4 domain from Botrytis cinerea (BcLOV4)
Source of Species
Botrytis cinerea
Class
LOV domain
GenBank
CCD53251.1
3D Structure
Components
Photoreceptor: LOV4 domain from Botrytis cinerea (BcLOV4)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Ras-related C3 botulinum substrate1
Detail Info
OS Application Information (1)
Mechanism of Action Intramolecular comformational change [ 1]
Controlled Signal Pathway Rac1 activation
Effect Sensor
Application Opto-control Rac1 activity to initiate actin polymerization and focal lamellipodia formation
Note BcLOV4 bind to anionic phospholipids only
Light Information Activation Wavelength 455 nm
Process Reversible
Deactivation Wavelength Thermal
Activation Time 5 s
Deactivation Time Seconds
Light Intensity 15 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation