Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00247
OS Name
Light regulated membrane location system (LRML)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV4 domain from Botrytis cinerea (BcLOV4)
Source of Species
Botrytis cinerea
Class
LOV domain
GenBank
CCD53251.1
3D Structure
Components
Photoreceptor: LOV4 domain from Botrytis cinerea (BcLOV4)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein LOV4 domain from Botrytis cinerea
Detail Info
OS Application Information (1)
Mechanism of Action Intramolecular comformational change [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Blue light dependent membrane localization in mammalian
Note BcLOV4 bind to anionic phospholipids but not zwitterionic phospholipid
Light Information Activation Wavelength 440 ± 20 nm
Process Reversible
Deactivation Wavelength Dark or thermal
Activation Time 1 s
Deactivation Time 18.5 ± 2.0 s
Light Intensity 15 milliwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines HEK293T cells
OS Application Information (2)
Mechanism of Action Intramolecular comformational change [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Blue light dependent membrane localization in fungus
Note BcLOV4 bind to anionic phospholipids but not zwitterionic phospholipid
Light Information Activation Wavelength 440 ± 20 nm
Process Reversible
Deactivation Wavelength Dark or thermal
Activation Time 1.2 s
Deactivation Time 84.9 s
Light Intensity 15 milliwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines Yesat
References
1 Directly light-regulated binding of RGS-LOV photoreceptors to anionic membrane phospholipids