Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00231
OS Name
Optogenetic control coffilin activity by LOVTRAP system 1 (OptoCOF-LOVTRAP 1)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
LOV2 Trapping of Protein (LOVTRAP)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
1047463129
PDB ID
5EFW
3D Structure
Components
Photoreceptor: LOV2 Trapping of Protein (LOVTRAP)
Complementary Protein: Zdark (ZDK)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Zdark (ZDK)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Cofilin; a actin-binding protein
Detail Info
OS Application Information (1)
Mechanism of Action AsLOV2-Zdk dissociation [ 1] , [ 2]
Controlled Signal Pathway Cofilin activation
Effect Sensor
Application Use Z-lock to opto-control cofilin activity in living cells
Light Information Activation Wavelength 445 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time <0.5 s
Deactivation Time Seconds
Light Intensity 0.064 nanowatts per square micron
Expression Information Expression Method Transfection
Cell Lines MTLn3 cells
References
1 Optogenetic control of cofilin and TAT in living cells using Z-lock
2 Engineering Optogenetic Protein Analogs