Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00228
|
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| OS Name |
LOV2 trap and release of protein system
(LOVTRAP)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Zdark (ZDK) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Functional protein | LOV2 domain of Avena sativa Phototropin 1 | ||||
| Recruited protein | Zdark | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | AsLOV2-Zdk dissociation | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | LOVTRAP-PEG Hydrogels for dynamic peptide immobilization a light responsive biomaterial | |||||
| Note | Zdk binds LOV2s dark state | |||||
| Light Information | Activation Wavelength | 450 nm or 480 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Thermal | |||||
| Activation Time | 240 s | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 14 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transformation | ||||
| Cell Lines | BL21 (DE3) E. coli cells | |||||