Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00204
OS Name
Optical dimerizar ssrA-cpLOV2 (CpLID)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of circular permutants (I539E) (cpLOV2 (I539E))
Source of Species
Circularly permuted from LOV2
Class
LOV domain
Mutation Site
I539E
3D Structure
Components
Photoreceptor: LOV2 domain of circular permutants (I539E) (cpLOV2 (I539E))
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein A bacterial SsrA peptide
Detail Info
Functional protein LOV2 domain of circular permutants
Detail Info
Recruited protein Stringent starvation protein B
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold and ssrA-SspB heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application A modular photoswitch for optogenetic engineering
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 4 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering