Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00175
OS Name
Photo-activable Rac1 system 2 (PA-Rac1-2)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (I539E) (AsLOV2 (I539E))
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
Mutation Site
I539E
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (I539E) (AsLOV2 (I539E))
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Ras-related C3 botulinum substrate1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Rac1 activation
Effect Activation
Application Elucidate Rac regulation of RhoA in cell motility
Note Mimics the unfold lit state
Light Information Activation Wavelength 458 nm or 473 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 A genetically encoded photoactivatable Rac controls the motility of living cells