Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00168
OS Name
Optogenetic modulation of REST target gene system 1 (Opto-REST 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Paired amphipathic helix 1
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Recruit endogenous mSin3a by diInterferes with the recruitment of endogenous mSin3a by direct competition for its binding site with REST
Effect Inhibition
Application Optogenetic inhibition of the RE1-silencing transcription factor to regulate gene transcription
Note mSin3 strategy to control transcription factor
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 12 h
Deactivation Time 12 h
Light Intensity 0.34 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Murine neuro2a neuroblastoma cells
References
1 Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor