Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00163
OS Name
Light-inducible Cre recombinase (LiCre)
 [1]
Photoreceptor (PR)
PR Info
PR Name
LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
162330142
PDB ID
2V1A
3D Structure
Components
Photoreceptor: LOV2 domain of Avena sativa Phototropin 1 (AsLOV2)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
OS Application Information (1)
Mechanism of Action J-alpha helix unfold [ 1]
Controlled Signal Pathway Recombinase activation
Effect Activation
Application Light inducible Cre recombinase to manipulate enzyme activity
Note Can be combined to control metabolic switches which are relevant to the problem of metabolic burden in bioprocess
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Minutes
Deactivation Time Tens of seconds
Light Intensity 3.63 milliwatts per square centimeter
Expression Information Expression Method Transformation
Cell Lines Flp-In T-REx 293 cells and yeast
References
1 A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch