Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00149
OS Name
Blue light-controlled gene expression system (B-GE)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Vivid PAS domain (VVD)
Source of Species
Neurospora crassa
Class
LOV domain
UniProt ID
Q9C3Y6
GenBank
149243087
PDB ID
2PDR
3D Structure
Components
Photoreceptor: Vivid PAS domain (VVD)
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Regulatory protein GAL4
Detail Info
OS Application Information (1)
Mechanism of Action VVD homodimerization [ 1] , [ 2]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Light controlled transcription activation in mammalian cells
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 3.5 h
Deactivation Time Hours
Light Intensity 1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells; U-2 OS cells; HEK293 cells; cos-7 cells;SH-SY5Y cells; Neuro-2a cells; NIH 3T3 cells
References
1 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
2 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells