Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00076
|
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| OS Name |
Light-switchable two-component system 1
(LSTCS 1)
|
[1], [2] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Chromatic acclimation regulator (CcaR) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Recruited protein | Chromatic acclimation regulator | ||||
| Functional protein | Chromatic acclimation sensor | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Autophosphorylates and transfer phosphoryl group to CcaR then activate transcripton from cpcG2 | [ 2] | ||||
| Controlled Signal Pathway | Transcription activation | |||||
| Effect | Activation | |||||
| Application | CcaSR-based light switch to refulate transcription in pseudomonas putida | |||||
| Light Information | Activation Wavelength | 520 nm | ||||
| Expression Information | Expression Method | Transformation | ||||
| Cell Lines | E.coli and Pseudomonas putida | |||||
| OS Application Information (2) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | Autophosphorylates and transfer phosphoryl group to CcaR then activate transcripton from cpcG2 | [ 1] | ||||
| Controlled Signal Pathway | Transcription activation | |||||
| Effect | Activation | |||||
| Application | Optimize bacterial TCSs to function as high-performance sensor to control gene expression | |||||
| Note | TCSs are the primary means by which bacteria sense and respond to their environment. | |||||
| Light Information | Activation Wavelength | 520 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 650 nm | |||||
| Activation Time | Hours | |||||
| Deactivation Time | Hours | |||||
| Light Intensity | 4.03 watts per square meter(green) 1.05 watts per square meter(red) | |||||
| Expression Information | Expression Method | Transformation | ||||
| Cell Lines | E. coli | |||||