Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00048
OS Name
Optogenetic controlled Focal Adhension Kinase (OptoFAK)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Focal adhesion kinase
Detail Info
OS Application Information (1)
Mechanism of Action CRY2 homodimeriization [ 1]
Controlled Signal Pathway FAK activation
Effect Activation
Application Optogenetic control focal adhesion kinase signaling
Note A valuable tool to modulate FAK activity
Light Information Activation Wavelength 451 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 10 min
Deactivation Time 30 min
Light Intensity 2 micromoles per square meter per second
Expression Information Expression Method Transfection
Cell Lines HEK 293T cells; HeLa cells and SC4 cells
References
1 Optogenetic control of focal adhesion kinase signaling