Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00043
OS Name
Optogenetic regulate RhoA activity by ARHGEF11 (OptoGEF-RhoA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein RhoA activator
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Control cellular forces and mechanotransduction
Note Should be readily applicable to study morphogenesis in animal embryos
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time <10 s
Deactivation Time 5 min
Light Intensity 4 milliwatts
Expression Information Expression Method Transfection
Cell Lines MDCK- cells
References
1 Optogenetic control of cellular forces and mechanotransduction