Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00040
OS Name
Optogenetic control FUS system (OptoFUS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein RNA-binding protein FUS
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHR homo-oligomeirization [ 1]
Controlled Signal Pathway Protein clust
Effect Activation
Application Elucidate physiological phase transitions and their link to pathological aggregates
Note Fully reversible even after multiple activation cycles
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 0.02-1.5 microwatts
Expression Information Expression Method Transfection
Cell Lines NIH 3T3 cells
References
1 Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets