Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00037
OS Name
Optogentic regulate stromal interaction molecule 1 system 3 (OptoSTIM1-3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Functional protein Cryptochrome-2
Detail Info
Recruited protein Stromal interaction molecule 1
Detail Info
OS Application Information (1)
Mechanism of Action CRY2PHRCCIBN heterodimerization [ 1]
Controlled Signal Pathway STIM1-gated Ca2+ influxion
Effect Activation
Application STIM1-gated Ca2+ influx to probe the molecular choreography
Note CRY2PHR acted as a photo-crosslinker to activate STIM1
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 23.4 ± 2.6 s
Deactivation Time 153 ± 26.2 s
Light Intensity 4.0 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling